摘要
采用Plaekett-Burman设计和响应面分析法,对皮状丝孢酵母菌发酵菊粉产油脂的培养基进行优化。首先用Plaekett-Burman设计对发酵培养基组分菊粉、酵母膏、硝酸钾、硫酸镁、硫酸铁、磷酸二氢钾、氯化钙、硫酸锌等进行评价并筛选出了有显著效应的酵母膏、硫酸锌、氯化钙,然后用最陡爬坡路径逼近最大响应值区域,最后再利用Box-Behnken试验设计及响应面分析法进行回归分析。结果表明酵母膏、硫酸锌、氯化钙于油脂含量存在显著的相关性。通过求解回归方程得到优化发酵培养基组分:酵母膏6.68g/L、硫酸锌0.457g/L、氯化钙0.342g/L。对优化后的培养基进行发酵培养,其生物量和油脂含量分别达到了13.56g/L、5.281g/L,油脂含量比优化之前增加了11.2%。
The use of Plaekett-Burman design and response surface analysis method for yeast fermentation to produce oil,optimize the culture medium,the first Plaekett-Burman design of fermentation medium components of inulin,yeast extract,magnesium sulfate,ferrous sulfate,potassium dihydrogen phosphate,calcium chloride,zinc sulfate were evaluated and selected has the remarkable effect of yeast extract,zinc sulfate,calcium chloride,and then use the path of steepest ascent approximation of the maximum response value,then the use of Box-Behnken area,experimental design and response surface analysis method for regression analysis.The results showed that yeast extract,zinc sulfate,calcium chloride in oil content had the remarkable correlation.By solving the regression equation to get the optimization of fermentation medium components： yeast extract 6.68g/L,zinc sulfate 0.457g/L,calcium chloride 0.342g/L.On the optimization of medium for fermentation culture,its biomass and grease content reached 13.56g/L,5.281g/L,fat content increased by 11.2% than before optimization.
出处
《中国酿造》
CAS
2012年第5期63-67,共5页
China Brewing
关键词
菊粉
皮状丝孢酵母菌
油脂
响应面分析
inulin
Trichosporon cutaneum
oil
response surface analysis
