山楂多糖的提取条件优化及对保加利亚乳杆菌的增殖影响

Optimization of hawthorn polysaccharide extraction condition and growth-promoting effect on Lactobacillus bulgaricus

采用热水浴法提取山楂多糖,正交设计优化提取条件,并将山楂多糖用于保加利亚乳杆菌的增殖试验。结果表明,山楂多糖的最佳提取条件为:料液比1∶20、提取时间6h、提取温度90℃。在此条件下,山楂多糖的提取率为3.92%。山楂多糖对保加利亚乳杆菌有促生作用并且在多糖添加量为2%,pH值为6.5,温度为42℃时促进效果最好。通过绘制生长曲线与普通培养基中生长曲线相比较,证明山楂多糖对保加利亚乳杆菌的生长有明显的促进作用。

The hawthorn polysaccharide was extraction with the method of hot bath,and was put into the growth test of Lactobacillus bulgaricus.And with the help of orthogonal experimental design,extraction condition was optimized.The results showed that with the optimized conditions,that solid-liquid ratio of 1： 20,time of 6h,the temperature at 90℃,the polysaccharide yield was up to 3.92%.The Hawthorn polysaccharide had growth-promoting effect on Lactobacillus bulgaricus most when polysaccharide ratio of 2%,pH value 6.5,temperature of 42℃.By comparing the growth curves,it showed hawthorn polysaccharide had obvious positive effect on Lactobacillus bulgaricus.