氮离子注入和微波复合诱变选育高产柠檬酸的黑曲霉研究

Study on the mutation breeding of Aspergillus niger fermented production citric Acid

利用低能离子注入及微波辐射对柠檬酸生产菌进行诱变选育。在30keV能量的氮离子注入以及间隔10s的微波电磁辐射后筛选得到2株产量提高到60%的诱变高产菌,多次传代实验表明菌株遗传稳定性良好,为柠檬酸发酵菌黑曲霉的进一步的育种工作提供了诱变参数和高产出发菌株。

The mutagenic effects of Aspergillus niger strains by low Energy ion implantation and microwave radiation had been studied.Through 30keV N＋ Implanting and interval 10s of microwave radiation on citric acid producing bacteria,two high-yield mutant strains were obtained,Their citric acid production ability was increased by about 60% than that of original.The results showed that the mutant strains had high genetic stability.It provided the mutagenic parameters and the high yield strain for the further breeding work of the citric acid fermentation byAspergillus niger.