油污土壤中脂肪酶产生菌的筛选

Screening of lipase producing strains in oil field

为了获得脂肪酶产生菌的菌种,进而获得高酶活菌株。通过添加橄榄油作为唯一碳源进行富集培养,然后以透明圈平板筛选法从富油土样中筛选出能够在溴甲酚紫培养基上形成黄色透明圈的菌落,再在罗丹明B的培养基上得到高活菌种以及水解圈大小,摇瓶复筛得高活菌株,进而测定酶的活力,经筛选有10株脂肪酶产生菌酶活力较高。

In order to obtain lipase-producing strain of the bacteria.Thus obtain high activity strains.By adding olive oil as the sole carbon source for enrichment culture,and then a transparent ring plate screening method to screen out from the soil samples can be formed on bromocresol purple medium yellow transparent circle colonies,and then cultured in Rhodamine B,Based on the live bacteria,as well as to get high hydrolysis ring size flask was re-screening high-living strains,and then measured the enzyme activity probably,there are four strains of lipase by screening bacteria produce higher enzyme activity.