载脂蛋白E基因多态性与子痫前期的相关性

Correlation Between Gene Polymorphism of Apolipoprotein E and Preeclampsia

目的探讨载脂蛋白E(ApoE)基因多态性与血脂水平、子痫前期(PE)发生的相关性。方法选取2007年1月至10月于广州医学院附属深圳沙井医院(深圳市宝安区沙井人民医院)住院分娩的孕产妇120例为研究对象,年龄为20～35岁。将其中诊断为PE者纳入PE组(n=50),其余正常妊娠孕妇纳入对照组(n=70)(本研究遵循的程序符合本院人体试验委员会制定的伦理学标准,得到该委员会批准,分组征得受试者的知情同意,并与其签订临床研究知情同意书)。两组孕妇的平均年龄及体重指数(BMI)比较,差异无统计学意义(P>0.05)。应用聚合酶链反应-限制性内切酶片段长度多态性(PCR-RFLP)检测受试者ApoE基因型及其血脂[总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL)-C、高密度脂蛋白(HDL)-C]水平,并进行统计学分析。结果 PE组孕妇血清TC,TG与LDL-C水平明显高于对照组,而HDL-C水平低于对照组,两组比较,差异均有统计学意义(P<0.05);PE组孕妇携带ApoEε3/4基因型者明显高于对照组,两组比较,差异有统计学意义(P<0.05);PE组孕妇携带ApoEε3/4基因型者脂代谢异常程度高于其他基因型。结论 ApoE基因多态性与PE孕妇存在脂代谢异常有关,可能为PE孕妇发病的遗传因素之一。

Objective To study the correlation of apolipoprotein E （ApoE） gene polymorphism, lipid levels and preeelampsia （PE）. Methods From January to October 2007, a total of 120 pregnant women, at the age of 20-35, were recruited into this study. Among them, 50 cases who were diagnosed as PE were included into PE group （n= 50）, the rest normal pregnant women were included into control group （n= 70）. The study protocol was approved by the Ethical Review Board of Investigation in Human Being of Shenzhen Shajing Affiliated Hospital of Guangzhou Medical College. Informed consent was obtained from each participate. There had no significant differences of average age and body mass index （BMI） between two groups （P〉0. 05）. The polymerase chain reaction-restriction enzyme fragment length polymorphism （PCR-RFLP） was used to analyze the ApoE genotypes. The lipid levels, including total cholesterol （TC）, triglyeride （TG）, low density lipoprotein （LDL）-C, high density lipoprotein （HDL）-C were analyzed. Results The levels of serum TC, TG and LDL-C in PE group were significantly higher than those in control group （P〈0.05）, however, the levels of HDL-C in PE group were much lower than those in control group （P〈0.05）. The ApoE ε3/4 genotype frequency in PE group was significantly higher than that in control group （P 〈0. 05）; Metabolic disorders were more serious in PE group with ApoE ε3/4 than other genotypes. Conclusions ApoE gene polymorphism was related to the abnormal lipid metabolism in PE patients, and may be a genetic one of factors of PE pathogenesis.