猪胰蛋白酶致大鼠COPD氧化应激与动脉血气间的相关性研究

The research of relationship between oxidatie stress and the changes of arterial blood gas in the rat with COPD induced by pig trypsin

目的探讨猪胰蛋白酶(PPE)致大鼠慢性阻塞性肺疾病(chronic obstructive pulmonary dis-ease,COPD)氧化应激与动脉血气变化的关系。方法 Wistar大鼠30只随机分为对照组(A组)、模型组(B组)和棉籽油组(C组),每组10只。B、C组大鼠气管内一次性注入PPE构建肺气肿模型,C组大鼠腹腔内注射棉籽油(1 ml/kg)。饲养30 d后处死解剖,测量大鼠肺体积,肺泡形态学,肺组织超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量和测定动脉血气分析,并用统计学方法探讨氧化应激与动脉血气间的相互关系。结果 B、C组大鼠肺体积、肺泡形态学、氧化应激水平均明显大于A组(P<0.05);B、C组肺组织SOD活力和MDA含量较A组显著升高(P均<0.05)。A组动脉血氧分压(PaO2)、动脉血氧饱和度(SaO2)、动脉血二氧化碳分压(PaCO2)基本正常;B、C组PaO2、SaO2明显降低,而PaCO2明显升高。肺组织SOD与PaO2呈正相关,肺组织MDA与PaO2呈负相关;肺组织SOD、MDA均与PaCO2不相关。结论用猪胰蛋白酶制作大鼠COPD模型成功,大鼠COPD氧化应激与动脉血气之间有着密切的相关性,值得在动物COPD实验造模中推广使用。

Objective To explore the research of relationship between oxidatie stress and the changes of arterial blood gas in the rat with COPD induced by pig trypsin （PPE）. Methods Thirty Wistar rats were randomly divided into control group （group A）, model group （group B）and cottonseed oil group （group C）, each group of each 10 rats. Established model in rats which rat＇s trachea injected for PPE,raising 30 days put to death, and the measurement of rat＇s lung volume, the alveolar morphology, content of SOD and MDA of lung tissue and determination of arterial blood gas analysis, and statistical methods to explore of relation oxidatie stress with arterial blood gas. Results The lung volume, the alveolar morphology, oxidative stress levels in group B and C were significantly greater than A group （ P 〈 O. 05 ） ; Content of SOD and MDA in B and C group of lung tissue were increased significantly than group A （ P 〈 0.05 ）. PaO2, SaO2 , PaCO2 in Group A was basic normal; PaO2 and SaO2 in B, C group were significantly reduced, and PaCO2 was increased significantly. SOD of tung tis- sue was positively associated with PaO2, MDA of lung tissue were negatively correlated PaO2 ; MDA and SOD of lung tissue were not related with PaCO2. Conclusion With pig trypsin make rat＇s model of COPD, the rats with COPD has close correlation between oxidation stress and arterial blood gas, the animal experiment mode of COPD is worthy of use of promotion.