党参粗提物对免疫青脚麻鸡血清抗体效价的影响

Effects of Lanceolata on the Serum Antibody Titer in Immune Cyan-shank Partridge Chicken

观察不同浓度的党参粗提物对青脚麻鸡血清中新城疫(ND)及传染性法氏囊炎(IBD)抗体效价的影响。400只1日龄青脚麻鸡随机均分4组,Ⅰ～Ⅳ组分别在饮水中添加0 g/L、0.5 g/L、1 g/L和2g/L的党参粗提物,14日龄开始给药,连续饮用6周,正常鸡新城疫和传染性法氏囊免疫。每周末各组随机抽样8只,心脏采血,分离血清,用微量血凝抑制实验(HI)测定ND抗体效价,琼脂扩散实验测定IBD抗体效价。结果表明,血清ND-HI抗体效价自28日龄起,IBD琼扩效价自35日龄起,党参各组较对照组均高出1～2个滴度以上,说明添加党参粗提物能够提高青脚麻鸡血清中ND-HI和IBD的抗体效价,其中中剂量药效为佳。

To observe the effects of Lanceolata crude extract with different concentration on the serum antibody ti- ter of Newcastle disease（ND） and Infections bursal disease（IBD） in Cyan - shank partridge chickens, 400 one day Cyan - shank partridge chichens were divided into four groups at random （50 per repetition）. The dosages of Lanceolata crude extract in I - 1Vgroups drinking water were respectively 0, 0.5, 1.0 and 2.0g/L from four- teen days old fed on Lanceolata for six weeks, ND and IBD antigen routine immunization. At each weekend, eight chickens were taken respectly in each group, from heart we picked the blood and separated the serum, and then serum antibody titer of ND and IBD were determined with Newcastle Hemagglutination Inhibition （ND - HI） and Agar Diffusion Experiment（ADE） respectively. The result showed that the ND -HI antibody titer from 28 days old and IBD antibody titer in Lanceolata groups were highter 1 to 2 titer than control group from 35 days old. It indicates that the adding Lanceolata crude extract can improve serum antibody titer of ND and IBD in Cyan - shank partridge chickens and moderate dose is better.