双孢蘑菇液体菌种培养基的优化

Optimization of Media for Agaricus Bisporus in Liquid Culture

采用正交试验优化双孢蘑菇的液体培养基配方,发现黄豆粉、葡萄糖、硫酸镁、以及玉米粉和葡萄糖的交互作用对双孢蘑菇的液体培养有显著影响,磷酸二氢钾、麦麸汁对实验结果无显著影响。双孢蘑菇最佳液体培养基配方为:黄豆粉2%,玉米粉2%,葡萄糖2%,磷酸二氢钾0.05%,硫酸镁0.20%,麦麸0.20%。把双孢蘑菇液体菌种接入小麦栽培种中,菌丝表现出生长快的优势,生长速度较为一致,满瓶时间短,证明利用液体菌种制备栽培种比固体菌种具有更大的优势。

According to the orthogonal experimental design method, the interaction was found between the maize meal and the glucose for Agaricus bisporus in liquid culture. The experiment has an optimum result when their ratio is three to two. The optimum prescription of the culture media could be ascertained as follows： soybean flour 2%, maize meal 2%, glucose 2%, KH：PO4 0.05% , MgS04 0.20% , and wheat bran 0.05%. The mushroom spawns of Agaricus bisporus grown in wheat kernels medium was displayed better characters inoculated with the liquid culture seed than the wheat kernels culture seed on superiority of quick growth, consistent growth speed of hypha and the full bottle time. It was concluded that this liquid culture may be used as suitable original seed for the mushroom spawns seed of Agaricus bisporus