光质对茶树愈伤组织中茶多酚及抗氧化酶活性的影响

Effect of Light Quality on Tea Polyphenol Content and Activities of Antioxidantive Enzymes in Tea Callus

以龙井43成熟茶籽为外植体诱导出的愈伤组织为材料，通过测定茶多酚含量（TP）及超氧化物歧化酶（SOD）和过氧化氢酶（cAT）等抗氧化酶活性的变化，研究了不同光质对次生代谢产物和酶活性的影响。结果表明，用不同的光质处理愈伤组织，愈伤组织中茶多酚含量、超氧化物歧化酶活性以及过氧化氢酶活性因光质和处理时间不同而异。蓝光处理下愈伤组织生长较好；各处理1-10d茶多酚含量增加，10-20d降低，蓝光处理下茶多酚含量低于对照，红光处理则高于对照，而绿光、黄光对其影响不显著；白光处理下SOD活性显著高于其余各组处理，红光处理下活性则显著低于各组处理；白光处理CAT的活性也显著高于其余各组处理，而绿光和黄光可能不利于CAT的合成。

Calli were induced from the mature seed of tea cultivar ＇Longjing 43＇ （C. sinensis cv. Longjing 43） using different light spectrum qualities. The effects of different lights on the tea polyphenol （TP） contents and the activities of antioxidantive enzymes （superoxide dismutase and catalase） in calli were analyzed. The results showed that the TP content, superoxide dismutase （SOD） activity and catalase （CAT） activity of the callus that treated with different light spectrum qualities varied with light quality and treatment time. Calli grew better under blue light than other treatments. The TP content of each treatment increased from 1-10 d and reduced from 10-20 d. The TP content under blue light was lower than the control while that under red light was much higher than the control, simultaneously green and yellow light had insignificant influence. The activity of SOD under white light was significantly higher than other treatments, while that of red light was significantly lower. The activity of CAT under white light was also significantly higher than other treatments. However, green and yellow light may not be conducive to the synthesis of CAT.