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联合应用ROCKII及GSK-3β抑制大鼠脊髓损伤后细胞凋亡的体内实验研究 被引量：3

Apoptosis inhibition with combined application of ROCKII and GSK-3β inhibitors in spinal cord injury in rats
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摘要 目的观察联合应用ROCKII和GSK-3β抑制剂对成体大鼠脊髓损伤后细胞凋亡的影响。方法①健康雌性SD成年大鼠80只,其中75只大鼠采用WD法制作成T10平面完全性截瘫的脊髓损伤动物模型,并在L4平面蛛网膜下腔植入注药导管后随机分为5组,每组15只,A组为TDZD-8治疗组,B组为Y27632治疗组,C组为TDZD-8+Y27632联合治疗组,D组为PBS对照组,E组为手术对照组;F组5只正常大鼠作为空白对照组。所用试剂均在手术后1h内经导管鞘内注射。②脊髓损伤后各组在8、24h和1w三个时间点,采用TUNEL法检测脊髓细胞凋亡情况。结果在各观察时间点,A组至E组平均凋亡细胞数均高于F组,A组至C组在各观察时间点均低于D、E两组,C组在各观察时间点均低于A、B两组,差异均有统计学意义(P<0.05)。结论 Y27632和TDZD-8均可减少脊髓损伤后细胞凋亡,联合应用比单独应用的作用更明显。 Objective To explore the combined apoptosis inhibition effect of ROCKII and GSK-3β inhibitors on spi- nal cord injury in rats. Methods 75 adult female Sprague-Dawley （SD） rats were randomly allocated into 5 groups. The complete paraplegia at T10 level was made by the weight-drop impaction. A catheter was set into subarachnoid space at L4 level of the rats. Group A was treated with the GSK-3βinhibitor TDZD-8. Group B was treated with the ROCKII in- hibitor Y27632. Group C was treated with the combination of TDZD-8 and Y27632. Group D was treated with PBS. Group E was sham operation group. The other 5 rats （group F） were as normal control. All the reagents were injected respectively into subarachnoid space within 1 hour after operation. The number of apoptotic cells in the spinal cord lesion of every group was assessed by TUNEL assay at 8 hours, 24hours and 1 week respectively. Results Compared with group A to group E, the average apoptotie cell number in group F was significantly less at all three time points. The av- erage apoptotie cell number in group A to group C was significantly less than the number in group D and group E respec- tively, and the significantly lowest cell number was observed in group c. The differences between group A and group B, group D and group E were not significant. Conclusion Compared with the individual application of ROCKII and GSK-3β inhibitor, Y27632 and TDZD-8 respectively, the combination is more effective in reducing the apoptosis after spinal cord injury, and is likely beneficial for the prevention of secondary damage after initial trauma.
作者 张戈 冯大雄 雷飞 周际
机构地区 泸州市中医院骨科 泸州医学院附属医院脊柱外科
出处 《西部医学》 2012年第6期1059-1062,1066,共5页 Medical Journal of West China
基金 国家自然科学基金资助项目(30872602)
关键词 脊髓损伤 RHO GSK-3Β 细胞凋亡 Spinal cord injury ROCK GSK-3β Apoptosis
分类号 R338 [医药卫生—人体生理学]
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共引文献4

同被引文献44

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西部医学
2012年 第6期

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