凹叶厚朴离体胚培养的研究

In vitro embryonic culture of Magnolia officinalis subsp.biloba

为了满足市场的资源需求,建立优质凹叶厚朴离体再生体系,为凹叶厚朴工厂化生产提供理论依据,以优质凹叶厚朴离体胚为外植体,以B5为基本培养基,6-BA、NAA、消毒时间为因素,采用L9(33)正交设计,进行无菌苗培养优化试验。结果表明:最佳消毒方法为0.1%升汞浸泡8 min,污染率为12.6%,外植体生长良好;最佳组合为B5+6-BA 0.5 mg/L+NAA 0.1 mg/L+消毒时间8 min,发芽率高达87%。

In order to satisfy the market＇s demands for the resources of Magnolia officinalis subsp, biloba （Rehd.et Wils.） Law., set up a high quality rapid propagation regeneration system of M. officinalis, and provide a theoretical basis for factory production, the optimization tests of aseptic seedling culture on M. officinalis have been carried out by using its embryos as in vitro culture material, adopting orthogonal design L9（3^4） method, and setting up 6-BA, NAA and disinfected time as the three main factors （every factor having 3 levels）. The results indicate that the best disinfect method was to sterilize the explants by mecruric chloridein （0.1%） for 8 minutes, and the optimum combination was B5 ＋ 6-BA 0.5 mg/L ＋ NAA 0.1 mg/L＋ 8 minutes （disinfection time）, its germination rate reached 87%.