香樟树新病原菌——二年残孔菌的形态学鉴定及ITS序列系统发育分析

A new pathogenic fungi of Cinnamomum camphora:morphological identification and ITS phylogenetic analysis of Abortiporus bienni

报道了香樟树的一种新病原菌,对采集的标本及其培养物的形态特征进行观察,对其ITS区序列进行测定和系统发育分析,将所测得的序列提交至Genbank获得序列号为JN182923。通过Blast比对获得同属近缘的序列后构建系统发育树并进行同源性分析。形态学鉴定和分子鉴定相结合将标本鉴定为二年残孔菌Abortiporus biennis,由系统发育树推断,二年残孔菌是多元进化的。

A new pathogenic fungi of Cinnamomum camphora was reported. The morphological characteristics and microscopic observation of the pathogen were conducted. The specimen＇s ITS rDNA sequence was submitted to Genbank and acquired a gene number JN182923. Through blast comparison we got the relative sequences and made homology analysis by constructed the phylogenetic tree. Combining traditional morphological identification and molecular identification,the ITS rDNA sequence were determined, and ITS rDNA system development was analyzed. The parasite was identified as Abortiporus biennis and the phylogenetic tree indicates that Abortiporus biennis is probably polyphyletic.