摘要
从草坪禾草根腐病病株不同发病部位分离到的病原菌,经致病性测定确定该病原菌为腐霉属的瓜果腐霉。本试验研究了不同温度、光照、pH值、碳氮源营养和多种培养基对瓜果腐霉菌菌落扩展、孢子囊产量和卵孢子产量的影响。结果表明,瓜果腐霉菌菌落扩展的温度范围为15~40℃,最佳温度为30~35℃,孢子囊产生的最适温度为15~25℃,卵孢子产生的最适温度为20~25℃。菌落在pH值为7~8的培养基上能够迅速扩展,孢子囊产量最大的pH值为9~10,pH值为6时卵孢子的产量最大。黑暗条件有利于菌丝生长和卵孢子的产生,光照条件下孢子囊产量最大,紫外光处理能加速菌丝的生长和孢子囊的产生,但同时却抑制了卵孢子的形成。淀粉对孢子囊和卵孢子的产生有明显的促进作用;瓜果腐霉可以很好的利用NH4NO3产生孢子囊,而脲有利于卵孢子的产生。
The chief pathogen was identified as Pythium aphanidermatum and its morphology,cultural characteristics and pathogenicity were investigated.The optimal temperatures for fungal growth were 30-35℃ but it could grow in the range 15-40℃.The temperature range for sporangium production was 15-25℃.The best pH was 7-8 for hyphal growth,9-10 for sporangium development,six for oospore development.The best cultural condition for mycelial and oospore growth was 24 h in the dark while the optimum treatment for sporangium growth was 24 h under light.Ultraviolet irradiation can accelerate the growth of hyphae and sporangia but it inhibits oospore production.The best source of carbon was amylum and the best source of nitrogen was ammonium nitrate.
出处
《草业学报》
CSCD
北大核心
2012年第3期93-98,共6页
Acta Prataculturae Sinica
基金
草业生态系统教育部省部共建重点实验室项目(CY-GG-2006-013)
甘肃省农牧厅生物技术专项(GNSW-2009-04)
甘肃省教育厅项目资助
关键词
根腐病
瓜果腐霉
生物学特性
root rot
Pythium aphanidermatum
biological characteristics