基于形态性状及Adh1基因序列的芒与五节芒自然杂交现象研究

Natural hybridization between Miscanthus sinensis and M.floridulus revealed by phylogenic analysis using morphological traits and Adh1 sequences

本研究通过对13份芒与五节芒17个形态性状及Adh1基因序列的分析,探讨了芒与五节芒的自然杂交现象。形态性状聚类分析结果表明,疑似杂交种AD431与五节芒类群(AA335和AD625)聚为一类,疑似杂交种AA343、AD431、AD628、AD607、AD633和AD606与芒类群(AD623、AD512、AD620、AD619和AD627)聚为一类。Adh1基因序列聚类分析结果表明,五节芒和芒的材料分别聚成2个明显分开的分支,所有的疑似杂交种中均检测到2种Adh1基因单倍型的存在,其中1个单倍型在系统树中与芒聚为一类,另1个单倍型与五节芒聚为一类。本研究的结果确证了AD431、AD431、AD628、AD607、AD633和AD606这6个疑似杂交种的真实性,证实了芒与五节芒种间确实存在自然杂交现象,为进一步阐明芒和五节芒的系统进化与亲缘关系提供了重要的理论依据。

Phylogenic analyses based on the morphological characters and Adh1 gene sequences were carried out to identify natural hybridization between Miscanthus sinensis and M.floridulus.One putative hybrid（AD431） clustered together with M.floridulus samples and other putative hybrids（AD623,AD512,AD620,AD619 and AD627） grouped with M.sinensis samples.However,two divergent types of Adh1 gene sequences were observed in all samples of putative hybrids,one of which clustered with M.sinensis while the other clustered with M.floridulus on the Adh1 phylogeny,suggesting that they are true hybrids between M.sinensis and M.floridulus.This study provides a basis for further research on genetic relationship between the two Miscanthus species.