等导数值导数光谱法测定万氏牛黄清心丸中黄连总生物碱的含量

Application of Equal Value Derivative Spectrophotometry to the Determination of Coptis Alkali in Wanshi Niuhuang Qingxin Pills

采用等导数值导数光谱法测定万氏牛黄清心丸中黄连总生物碱的含量。该法通过索氏提取器简单提取,以盐酸小檗碱为标准对照,便可直接测定,方法可靠、稳定、灵敏度高。其平均回收率为98.55±1.90%。

The total coptis alkaloids Wanshi Niuhang Qingxin Pills were determined by equal value derivative spectrophotometry in this paper. Quantitative analysis of six mixtures of medicinal herbs can be done easily on the extract. The average recovery of berberine. HCl is 98.55±1.90% (CV). The method features good reproducibility, favorable stability and high sensitivity.