气质联用技术分析测定啤酒花中脂肪酸

Determination of Fatty Acids in Hops by Gas Chromatography-Mass Spectrometry

对啤酒花中的脂肪酸类化学成分进行分析测定。采用索氏提取法对啤酒花样品中脂肪酸进行提取,运用气质联用技术结合计算机检索对提取物甲酯化的化学组成及含量进行测定分析。结果表明,啤酒花中主要含有棕榈酸甲酯、亚麻酸甲酯、亚油酸甲酯、油酸甲酯、硬脂酸甲酯等22种脂肪酸;用面积归一化法测定各种脂肪酸相对含量,其中不饱和脂肪酸亚麻酸与亚油酸含量超过70%。

The chemical compositions and their relative content of fatty acids from hops were analyzed and measured. Fatty acids from hops were extracted by Soxhlet, then their chemical compositions was analyzed by GC-MS combining with computer search. Finally, 22 kinds of fatty acids from hops were identified including methyl palmitate, methyl linolenate, methyl oleate, and methyl stearate etc. The relative content of each kind of fatty acid was determined by peak area normalization method, and the content of unsaturated fatty acid and linolenie acid was more than 70 %.