HPLC测定栀龙散中的栀子苷

Determination of Geniposide in Zhilong Culvis by HPLC

目的:建立以高效液相色谱法测定栀龙散中的栀子苷。方法:采用高效液相色谱法,色谱柱为Diamonsil C18(4.6mm×200mm,5μm);流动相为乙腈∶水(15∶85);检测波长为238nm,流速1.0ml·min-1;柱温25℃;结果:栀子苷在0.18～1.80μg范围内与峰面积线性关系良好(r=0.9999),平均加样回收率为98.42%,RSD=1.63%。结论:该质量检测方法专属性强,可用作栀龙散的质量控制。

Objective：To establish a HPLC method to determine the geniposide in zhilong powders. Methods：Diamonsil C18 （4.6nm× 200nm, 5μm） was used.The HPLC system consiste of acetonitrile-water （15：85）mixture as a mobile phase, detecting wavelength was set at 238nm.The flow rate was 1.0ml·min-1 and column temperaturewas at 25℃. Results： Winthinthe range of 0.18-1.80μg,there was a good linear relationship between the concentration and peak arer of geniposide （r=0.9999）.the average recovery was 98.42%, with RSD of 1.63%. Conclusions：The method is accurate, reliable,it can be used in the quality cobtrol of Zhilong powders.