摘要
目的探讨血管内皮生长因子(VEGF)、微血管密度(MVD)和趋化因子受体CXCR-4基因在结肠恶性病变中的表达及意义。方法收集经手术切除且病理证实的结肠癌病例68例,所有患者术前均未经放疗或化疗,记录各患者肿瘤部位,组织学类型,病理分期等临床资料,采用免疫组织化学法检测标本中VEGF、CX-CR-4和MVD的表达。结果 VEGF,CXCR-4和MVD表达与患者的分化程度、组织学类型无关(P>0.05)。VEGF,CXCR-4和MVD表达与结肠癌临床分期和淋巴结转移差异有统计学意义(P<0.05)。结论三者联合检测可能有助于结肠癌的病理学分期诊断。
Objective To investigate the expression of vascular endothelial growth factor （VEGF）, mi- crovessel density （MVD） and the CXCR-4 chemokine receptor gene in malignant lesions of the colon cancer and their significance. Methods 68 cases of colon cancer cases confirmed by surgery and pathology are enrolled. All patients with preoperative radiotherapy or chemotherapy. Clinical data for tumor location, histological type, path- ological stage of patients were recorded. Immunohistochemical assay was used to analyze the expression of VEGF, CXCR-4 and MVD. Results VEGF, CXCR-4 and MVD in patients are not correlated with the degree of differenti ation and histological type （P〉0.05）. VEGF, MVD and CXCR-4 in patients with colon cancer are significantly correlated with clinical stage and lymph node metastasis （P〈0.05）. Conclusion Joint detection of VEGF, MVD and CXCR-4 may contribute to pathological staging in colon cancer.
基金
湖南省科学技术厅基金(B2011-065)
