H9N2亚型禽流感安徽分离株CK/AH/1/10的分子特征

Molecular Characteristics of an H9N2 Avian Influenza Virus CK/AH/1/10 Isolated in Anhui Province

为了了解H9N2亚型禽流感病毒安徽分离株CK/AH/1/10基因组的分子特征,本研究采用RT-PCR方法扩增了CK/AH/1/10分离株8个片段的基因,克隆测序后进行同源性比较及遗传进化分析。结果显示:分离株CK/AH/1/10 HA蛋白的裂解位点为低致病性禽流感病毒,参照Guan等[4]对H9亚型禽流感病毒HA基因的分类标准,可将CK/AH/1/10株归属于欧亚系G9-like亚分支。

In 1994,for the first time,H9N2 virus was isolated from diseased chickens in Guangdong Province,China.Since then,this subtype of influenza virus has been found in domestic poultry in other provinces in China.An H9N2 virus,named as CK/AH/1/10,was isolated in Anhui Province,China.To evaluate the molecular characteristics of this H9N2 isolate,eight full-length genes of CK/AH/1/10 isolate were amplified by means of RT-PCR.The products of RT-PCR were cloned into pMD-18 T vectors and sequenced.Sequence analysis indicated CK/AH/1/10 possessed few basic amino acids at the HA cleavage site and it was classified as low pathogenic avian influenza virus.According to Guan[4],CK/AH/1/10 could be classified in G9-like sublineage in the Eurasian lineage,based on the characteristics of HA gene determined by phylogenetic analysis.