小麦杂交F_1和双亲幼苗动态蛋白质组分析

Dynamic Changes of Leaves Proteome Between Wheat F_1-hybrid and Its Parental Lines

分别以2,3,5 d叶龄的栽培小麦京411、偏硬001和杂交F1幼苗为材料,利用动态蛋白质组技术,对F1及双亲的蛋白质变化做了比较分析,结果表明,在双亲及F1中蛋白质差异表达现象被观察到,其中在5 d的叶子中最为明显。如在发育2 d和3 d的F1叶子中,各有15个蛋白质斑点的含量产生变化,而在5 d叶龄的叶子中,这种有变化的蛋白质斑点达27个,在这些变化的蛋白质中,偏双亲和低于双亲的为多数,如5 d的叶子中分别占41%和44%,同时,在F1叶片中观察到有蛋白质消失和新的蛋白质诱导现象产生。这些结果表明,杂种中存在蛋白质的差异表达现象,展开这方面的研究有助于对杂种优势形成机理的理解。

In this study,proteome approaches were applied to a comparative analysis of hybridization in wheat F1-hybrid and its parent lines by using 2,3,5 d leaves.The results indicated that protein expression differences were observed between F1-hybrid and parents.In the 5 d leaves,27 protein spots were changed in the protein abundances and only 15 protein spots were changed in the protein abundances at 2 d,3 d leaves in F1 hybrid.In these changed proteins,most protein spots were showed ＂two parental lines＂characteration（41%） and ＂low parents＂characteration（44%） in protein abundances.we also observed the some protein sports were lost and some new protein spots were induced in the F1-hybrid.These results indicated that hybridization between two parental lines could cause expression differences between wheat hybrid and its parents at protein abundances.