摘要
以普通玉米自交系8984与高油玉米自交系GY220为亲本组配得到284个F2:3家系群体,利用185个SSR标记构建玉米遗传连锁图谱。通过包含母体效应的种子性状QTL作图方法对玉米籽粒淀粉含量进行QTL定位和效应分析,共检测到7个与淀粉含量相关的QTL,分别位于第5,8,10染色体上,除qSTA8-3的遗传作用方式表现为加性外,其余QTL作用方式为部分显性。单个QTL贡献率为5.87%～10.93%,累计贡献率为53.37%。所有QTL的增效基因均来自普通玉米亲本8984。淀粉含量QTL qSTA5-2贡献率较大,可以作为进一步精细定位的主要目标QTL。
F2：3 populations consisted of 284 plants were developed from the cross between a normal maize inbred 8984 and a high-oil maize inbred GY220,and a genetic linkage map including 185 SSR markers constructed by（8984/GY220） F2 population.The quantitative trait loci（QTL） of starch content were identified and their effects were evaluated using SeedTraitQTL containing maternal effect.A total of seven QTL were detected in the F2：3 population,located on chromosome 5,8 and 10 respectively.Six QTLs showed partially dominant effects but qSTA8-3 showed additive.Contribution of single QTL to phenotypic variation varied from 5.87% to 10.93%,and total contributions of all QTLs were 53.38%.The allele of all QTLs were contributed by the normal maize parent 8984.The QTL qSTA5-2 with higher contribution could be used as the main objective QTL in fine mapping.
出处
《华北农学报》
CSCD
北大核心
2012年第2期97-99,共3页
Acta Agriculturae Boreali-Sinica
基金
河南省科技攻关项目(0424020025)
