CPPU对干旱胁迫下小麦幼苗叶片的蛋白响应及生育期差异的影响

Effects of CPPU on Protein Response of Wheat Seedlings Leaves and Different Growth Stage under Drought Stress

采用叶面喷施调吡脲(N-(2-chloro-4-pyridyl)-N'-phenylurea,CPPU)处理,分析干旱胁迫下CPPU对小麦幼苗叶片蛋白质及各种生理生化指标的影响,以探讨CPPU提高小麦抗旱性的分子机制。以小麦品种河农822为材料,用聚丙烯酰胺凝胶电泳法(SDS-PAGE)研究喷施CPPU后幼苗叶片中蛋白质组变化情况;用常规方法检测过氧化物酶(POD)活性、可溶性蛋白、可溶性糖、脯氨酸和丙二醛(MDA)含量的变化。结果表明,经SDS-PAGE电泳技术发现,与对照相比,干旱胁迫下喷施CPPU后出现分子量为125,80,55,40,35,33,25 kDa等7条特异性条带,消失了30 kDa的电泳条带。与干旱对照相比,喷施CPPU后叶片中蛋白条带中多了40 kDa特异性条带,消失了30 kDa的电泳条带;并且在干旱胁迫下,喷施100 mg CPPU后使幼苗体内的POD活性提高37.06%,可溶性蛋白含量增加63.50%,可溶性糖含量增加87.51%,脯氨酸含量增加109.57%,MDA含量降低46.06%。与干旱对照相比,喷施CPPU的幼苗叶片中出现了一条特异性条带,消失了一条特异性条带,且在70 kDa和大约20 kDa处的蛋白含量明显高于2个对照,在32kDa和大约25 kDa处的蛋白含量下降。100 mg CPPU可促进干旱胁迫下幼苗体内的可溶性糖、可溶性蛋白和脯氨酸含量显著增加,POD活性显著提高,MDA含量降低。说明叶面喷施CPPU能够增强幼苗对干旱胁迫的适应性。

This experimentation focused on the effects of CPPU on wheat seedlings proteins and physiology-bio- chemistry indexes under drought stress to explore the molecular mechanisms of CPPU treatment on improving drought resistance of wheat by foliage spraying with CPPU （N-（2-chloro-4-pyridyl）-N-phenylurea）. With Henong 822 as materials,SDS-PAGE was used to study changes of proteomics in seedlings leaves,and routine methods were used to test changes of peroxidase （POD） activity, the contents of soluble sugar and protein, proline and malondi- aldedyde （MDA） contents. The result of SDS-PAGE showed that seven specific electrophoresis bands were obtained after CPPU treatmant compared with the control under drought stress, their molecular weight was 125,80,55,40, 35,33 and 25 kDa respectively,but one of 30 kDa was disappeared. In compared with the drought control,40 kDa specific band was found in protein bands of leaves with CPPU treatment,but 30 kDa strip was disappeared. The pro- tein contents of 70 kDa and about 20 kDa were higher than two contrast groups,while the protein contents of 32 kDa and about 25 kDa were decreased. After spraying 100 mg CPPU,POD activity was increased by 37.06v/o ,the con- tents of soluble protein and sugar were improved by 63.50% and 87.51% ,proline content was improved by 109. 57% ,but MDA content was decreased by 46.06% in wheat seedlings under drought stress. It illustrated spraying CPPU on leaves could strengthen adaptability of wheat under drought stress. In compared with the drought control,one specific protein band was found in seedlings leaves of treatment with CPPU,one was disappeared and the pro- tein contents of 70 kDa and about 25 kDa were higher than two contrast groups,while the protein contents of 32 kDa and about 25 kDa were decreased. 100 mg of CPPU could improve the contents of soluble sugar,protein and proline as well as POD activity significantly,but decrease MDA content in wheat seedlings under drought stress. It illustra- ted spraying CPPU on leaves could strengthen adaptability of seedlings under drought stress.