分子标记选择水稻抗稻瘟病基因Pi40和Pib聚合体

Selecting the Pyramids of Blast Resistance Genes Pi40 and Pib in Rice Assisted by Molecular Marker

培育多个抗病基因聚合的水稻品种是提高其抗病广谱性和持久性的有效途径之一。利用水稻抗稻瘟病基因Pi40、Pib的特异PCR标记,对含有Pi40、Pib的水稻品系复合杂交F250个植株进行检测,从中选择到Pi40、Pib双基因聚合的抗病植株28个,为持久、广谱抗病水稻育种奠定了基础。

Pyramiding resistance genes have been suggested as one of the most effective methods for proventing breakdown of major gene conferring resistance to blast within a very short period.The PCR markers of the blast resistence gene Pi40 and Pib were used to identify the genes in F2 plants of combination among the rice lines containing Pi40 and Pib respectively.The results indicated that all the plants containing any one gene,two genes of Pi40 and Pib showed resistane to blast,while the plants lacking the resistance gene were susceptible to blast.The tightly-linked markers of the resistance genes were powerful tools for the identification of Pi40 and Pib in breeding program.28 plants pyramiding two genes of Pi40 and Pib were selected from 50 plants of the F2 population.They can be used as parents to breed rice for blast resistance.