复方制剂补血安神胶囊中五味子醇甲含量的研究

Determination of Schizandrol A in Buxue'anshen Capsule

建立补血安神胶囊质量控制方法。采用HPLC法测定补血安神胶囊中五味子醇甲的含量,色谱柱C18-ODS(150×4.6mm)、流动相V甲醇:V水=65:35、流速1.0mL/min、检测波长250nm、柱温30℃。五味子醇甲在0.132μg^0.528μg范围内峰面积与含量呈良好的线性关系,回归方程:Y=2 484 056.277X-9 102.857 1,且r=0.999 9;精密度RSD=0.73%,重现性RSD=2.41%,稳定性RSD=0.49%,回收率平均值为98.74%,RSD=2.62%(n=5)。本方法对补血安神胶囊复方制剂中的五味子醇甲含量测定适用、可行,并且可将通过HPLC法测定五味子醇甲含量的方法作为控制本复方制剂的质量标准。

To establish a method for the determination of schizandrol A in Buxue＇ anshen capsule. Method： Using C18-ODS （150 × 4.6mm） ,VmethanohVwater=-65：35, 250nm detection wavelength, 1.0mL/min flow rate, 30℃ column temperature. There was a good linear relationship between the concentration of schizandrol A and absorption area value in the range of 0.132-0.528μ g. The regression equation was Y=2 484 056.277X-9 102.857 1, the correlation coefficient was 0.999 9, the precision was 0.73%, the HPLC method showed good repeatability （RSD=2.41%） and stability （RSD=0.49%）, the mean recovery rate was 98.74% （RSD=2.62%, n=5）. RP-HPLC method is sensitive, simple, accurate and suitable for the determination of Schizandrol A in Buxue＇ anshen capsule.