青蒿琥酯对腺样囊性癌NACC细胞系抑制作用的实验研究

Inhibition Effects of Artesunate on Novel Human Palatal Salivary Gland Cell Line of Adenoid Cystic Carcinoma in vitro

目的观察青蒿琥酯(ART)对人腭部小涎腺腺样囊性癌(NACC)细胞体外增殖抑制和诱导凋亡作用,并探讨其机制。方法经不同浓度ART处理体外NACC细胞株后,噻唑蓝法、克隆形成实验检测ART对细胞增殖的影响,并在倒置显微镜及电镜下观察细胞形态学的改变;细胞划痕实验检测ART对细胞迁移能力的改变;流式细胞仪检测ART对NACC细胞周期及凋亡的影响。结果 (1)ART对体外NACC细胞增殖有明显抑制作用(P<0.05),这种作用呈明显的时间、剂量依赖性(P<0.05),24 h、48 h、72 h ART半抑制浓度分别为113.09、74.80、35.61μg/ml;(2)ART处理后的NACC细胞出现形态和结构的改变;(3)ART可以明显地抑制NACC细胞的迁移(P<0.05);(4)不同浓度ART均能促进NACC细胞凋亡,随着ART浓度的增加,NACC细胞凋亡率逐渐升高(P<0.05);(5)低浓度ART可将细胞阻滞在G1期,当浓度达到50.0μg/ml时,ART可将细胞阻滞于G2期。结论 ART对人腺样囊性癌NACC细胞具有明显的抑制作用,并能调整细胞周期,诱导其凋亡。

Objective To investigate the apoptosis and cell growth inhibition effects in vitro of artesunate （ART） on novel human palatal salivary gland cell line of adenoid cystic carcinoma（NACC）,and to explore the underlying mechanism of this action. Methods Cell growth inhibition ratio was determined by the methyl thiazolyl tetrazolium（MTT） method and clone formation experiment in vitro after NACC cell line were treated with various concentrations of ART,then observe their morphological changes by inverted microscopy and electron microscopy. The effects of ART on cell migration ability of NACC cells were detected by wound healing method,and flow cytometry（FCM） assay was used to analyze the distributions of ceU cycles and cell apoptosis. Results （1） The proliferation of NACC cell line was significantly suppressed by ART in vitro in a timeand dose-dependent manner（P 〈0.05）. Half maximal inhibitory concentration（IC50 ） of ART in 24,48 and 72 h were 113.09,74. 80,35.61 μg/ml,respectively. （2） ART could change the morphological structure of NACC cells. （3） ART significantly inhibited the NACC cell migration（P 〈0.05）. （4） ART could induce the apoptosis of NACC cells at various concentrations. With the increase of concentration of ART, apoptosis ratio of NACC cell was （P 〈0.05）. （5）The cells were arrested in G, phase by ART at low concentration and in G2 phase Conclusion ART can significantly inhibit the growth of NACC cell in vitro, and adjust the cell cycle apoptosis. also elevated at 50 ~g/ml. and induce its