摘要
以‘高原7号’马铃薯茎尖为外植体,用0.1%升汞和75%酒精消毒,经组织培养获得脱毒马铃薯茎段,在增殖培养中,研究不同浓度6-BA及KT对马铃薯茎段诱导增殖效果的影响。结果表明:用0.1%升汞对茎尖消毒8 min效果最好,灭菌率达96.67%。细胞分裂素对马铃薯茎段增殖的促进作用为6-BA>KT,以1.5 mg/L6-BA诱导不定芽及其生长势效果最佳。
Shoot tips of ＇Highland No.7＇ potato are selected as explants and 0.1% Mercuric Chloride and 75% alcohol are employed to disinfect; then through tissue culture, virus-flee potato stems are obtained. In the process of enrichment culture, the paper tries to analyze the effect that 6-BA and KT with different consistency have on proliferation-inducing result of potato stems. The result indicates that it is the most effective to use 0.1% Mercuric Chloride to disinfect the shoot tips for 8 minutes, by which the disinfection rate can be up to 96.67%. The better promoting effect of potato stem proliferation made by cytokinin can be achieved when 6-BA 〉 KT; adventitious bud induction and growth potential are the best when the consistency of 6-BA reaches 1.5 mg/L.
出处
《亚热带植物科学》
2012年第2期19-22,共4页
Subtropical Plant Science
基金
安徽省高等学校省级自然科学研究项目(KJ2009B064Z)
淮北师范大学校青年项目(700295)资助
