针刺留针与否对大鼠脑缺血再灌注后白细胞浸润的影响

Influence of Retained Needling and Non-retained Needling on Leukocyte Infiltration in Brain Tissue of Cerebral Ischemia and Reperfusion Model Rats

目的:研究针刺留针与否对局灶性脑缺血再灌注大鼠脑组织中白细胞浸润及神经组织缺血程度的影响。方法:40只SD大鼠,随机分为正常组、假手术组、模型组、针刺留针组及针刺不留针组。针刺留针组在造模后2 h、24 h针刺三阴交、内关、人中,留针30 min;针刺不留针组针刺时间、穴位与针刺留针组相同,针刺后即起针。造模48 h后,取脑固定后行HE染色,比较各组大鼠浸润白细胞计数和神经组织缺血程度。结果:针刺留针组与针刺不留针组浸润白细胞数均低于模型组,差异有统计学意义(P<0.05);神经组织缺血程度较模型组轻,差异无统计学意义(P>0.05)。针刺留针组与针刺不留针组脑组织中浸润白细胞数及神经组织缺血程度比较差异无统计学意义(P>0.05)。结论:针刺能明显减少脑缺血坏死周边区的白细胞浸润,减轻神经细胞缺血程度。

Objective：To observe the influence of retained needling and non-retained needling on leukocyte infiltration and isehemia extent of nerve tissue in brain tissue of rats with cerebral ischemia and reperfusion. Methods：40 male SD rats were randomly divided into normal group,sham operation group,model group,retained needling group and nonretained needling group. Rats in retained needling group were acupunetured at Sanyinjiao,Neiguan and Renzhong 2 hours and 24 hours after models were constructed,then the needles were retained for 30 minutes. Rats in non-retained needling group were aeupunetured at the same point. 48 h after models were constructed,brain of rats were taken out and fixed, then were underwent HE staining. At last,numbers of leukocyte infiltration and isehemia extent of nerve tissue were observed. Results ：Compared with model group,numbers of leukocyte infiltration were decreased （P〈0.05）and ischemia degree of nerve tissue in acupuncture groups was decreased,too （P〉0.05）.Difference of leukocyte infiltration and ischemia extent of nerve tissue between retained needling group and non-retained needling group was not statistically significant（P〉 0.05 ）. Conclusions ： Acupuncture can significantly decrease leukocyte infiltration of surrounding area of cerebral isehemia, and reduce ischemia extent of nerve tissue.