超滤和纳滤集成膜技术纯化蒸汽爆破秸秆木聚糖酶酶解液

Purification of Hydrolysate of Steam-Exploded Wheat Straw by Xylanase Using Integrated Membrane Technology of Ultrafiltration and Nanofiltration

为掌握蒸汽爆破秸秆木聚糖酶酶解液的超滤脱色和纳滤浓缩的集成膜技术,研究了两支不同截留相对分子质量的超滤膜脱除色素的工艺,测定了膜通量数据并取样检测色值和还原糖含量,得到脱色率和还原糖回收率,优选一支超滤膜用于脱色;采用截留相对分子质量为150的纳滤膜浓缩超滤工艺的透过液,提高糖液浓度.集成膜工艺的脱色率达到63.6%(对原料液的色值),总还原糖回收率达到92.1%(对原料液的还原糖),说明超滤和纳滤集成膜技术可以脱除蒸汽爆破秸秆木聚糖酶酶解液内的色素,提高糖液的浓度,并且膜通量较高.

The integrated membrane technology of uhrafiltration（UF） and nanofiltration（NF） were used for purification of the hydrolysate of steam-exploded wheat straw by xylanase with various molecular weight cut-offs （MWCO）. The reducing sugar concentrations and color values were collected, and decolorization rates and reducing sugar recovery were obtained. Then, an optimum UF membrane was selected for de- colorization. The permeate of UF was concentrated by NF with MWCO 150 Dalton. Decolorization rate reached 63.6% （on color value in feed） and total reducing sugar recovery（on reducing sugar concentra- tion in feed） was 92.1% by integrated membrane technology, which indicate that integrated membrane technology of UF and NF can decolor and concentrate hydrolysate of steam - exploded wheat straw by xy- lanase. With high membrane flux, the integrated membrane technology may have industry application prospect.