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心房钠尿肽对力竭小鼠心脏抗氧化作用及其分泌机制的研究

Study on mechanism of ANP release and cardiac antioxidation on exhausted heart
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摘要 目的观察游泳力竭小鼠心肌组织中乳酸(lactic acid,LD)含量及血浆心房钠尿肽(atria1 natriuretic peptide,ANP)的分泌,以探讨力竭心脏的ANP分泌作用及抗氧化作用机制。方法①对小鼠进行分组及负重游泳,力竭即测试心肌LD和血浆ANP含量;②采用家兔离体心房灌流模型,利用不同浓度的乳酸缓冲液(4、8和12 mmol/L)及其氮气模拟力竭心脏,探讨力竭心脏分泌ANP的机制;③利用氮气复制缺氧家兔离体心房灌流模型模拟力竭心脏,观察力竭心肌MDA含量的变化及其机制探讨。结果①游泳力竭小鼠心肌组织LD(P<0.01)和血浆ANP含量(P<0.01)与对照组相比均明显增高;②灌流浓度分别为4 mmol/L(P<0.05)、8 mmol/L(P<0.05)和12mmol/L(P<0.001)的乳酸缓冲液与对照组相比并不增加离体心房灌流模型中ANP的分泌,反而抑制ANP的分泌;但缺氧能明显增加心房ANP的释放(与对照组相比,P<0.001);③缺氧的离体心房灌流模型显示,缺氧组心肌组织中MDA含量比对照组显著降低(P<0.05),而预处理KT5823(1 mmol/L)再给予缺氧组心肌组织中MDA含量明显增高(与缺氧组相比,P<0.01)。结论①力竭心脏的缺氧分泌大量ANP,而不是乳酸。②力竭时分泌的ANP对心脏具有抗氧化作用,其作用是通过ANP→cGMP→PKG信号传导途径。 【Objectives】 To observe the secretion of the ANP and study the cardiac antioxidation and mechanism of ANP release on exhausted heart.【Methods】 Experimental animals were grouped and swimmed,the exhausted mouse was tested the lactic acid(LD) and ANP in blood plasma;To observe the effect of hypoxia and LD on ANP secretion in the exhausted heart by the isolated perfused beating rabbit atria;To observed the MDA content in the exhausted myocardium which pretreatment and non-pretreatment KT5823(1 mmol/L)(PKG inhibiter) by isolated perfused beating rabbit atria.【Results】 The LD in myocardium(P 〈0.01) and ANP in plasma(P 〈0.01) significantly increased of swimming-induced exhausted mouse compaired with control group.Hypoxia significantly increased atrial ANP secretion(P 〈0.001 vs control cycle) but not latic acid in the isolated perfused beating rabbit atria;The MDA content in hypoxic myocardium significantly decreased(P 〈0.05) and this effects blocked by KT5823,an inhibiter of protein kinase G(P 〈0.01 vs hypoxia).【Conclusion】 ANP was increase by hypoxia but not latic acid in exhausted heart;The antioxidation of ANP on hypoxic myocardium through ANP→cGMP→PKG pathway.
出处 《中国现代医学杂志》 CAS CSCD 北大核心 2012年第14期5-8,共4页 China Journal of Modern Medicine
基金 国家自然科学基金(No:30860091)
关键词 心房钠尿肽((ANP) 力竭 缺氧 乳酸 抗氧化 ANP exhausted hpoxia latic acid antioxidation
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  • 1王福文,胡志力,李杰.力竭性运动致运动性心肌损伤的产生机制[J].中国临床康复,2005,9(8):144-146. 被引量:41
  • 2李辉,杨青,张继峰,唐朝枢,赵云涛,石湘芸.心钠素对内皮素某些生物学效应的拮抗作用[J].中国病理生理杂志,1994,10(2):169-172. 被引量:13
  • 3de Bold AJ, Borenstein HB, Veress AT, et al. A rapid and potent natriuretic response to intravenous injection of atrial myocardial extracts in rats [J]. Life Sci, 1981,28( 1 ) :89-94.
  • 4Shono M, Yoshimura M, Nakayama M, et al. Predominant Effect of A-Type Natriuretic Peptide on Reduction of Oxidative Stress During the Treatment of Patients With Heart Failure [ J ]. Circ J, 2007,71 (7) : 1040-1046.
  • 5Laskowski A, Woodman OL, Cao AH, et al. Antioxidant actions contribute to the antihypertrophic effects of atrial natriuretie peptide in neonatal rat eardiomyocytes[ J ]. Cardiovasc Res, 2006,72 (1) : 112-123.
  • 6Cho KW, Kim SH, Kim CH, et al. Mechanism basis of atrial natriuretic peptide secretion in beating atria: atrial stroke volume and ECF translocation[ J 1. Am J Physiol, 1995,268 ( 5 pt 2 ) : 1129- 1136.
  • 7Ftirst R, Brueckl C, Kuebler WM, et al. Atrial Natriuretic Peptide Induces Mitogen-Activated Protein Kinase Phosphatase-1 in Human Endothelial Cells via Racl and NAD(P) H Oxidase/Nox2-Activation [ J]. Circ Res, 2005,96( 1 ) :43-53.
  • 8Edwards JG. Swim training increases ventricular atrial natriuretic factor (ANF) gene expression as an early adaptation to chronic exercise [ J ]. Life Sci, 2002,70 ( 23 ) :2755-2768.
  • 9Gutkowska J, Paquette A, Wang D, et al. Effect of exercise trraining on cardiac oxytocin and natriuretic peptide systems in ovariectomized rats[J]. Am J Physiol Regul Integr Comp Physiol, 2007,293 ( 1 ) : R267-R275.
  • 10Calderone A, Thaik CM, Takahashi N, et al. Nitric oxide, atrial natriuretic peptide, and cyclic GMP inhibit the growth-promoting effects of norepinephrine in cardiac myocytes and fibroblasts [ J 1. J Clin Invest, 1998,101 (4) :812-818.

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