大黄对脓毒症大鼠肠道菌群紊乱影响的实验研究

Effects of Chinese rhubarb on intestinal flora disturbance of septic rats

目的探讨中药大黄对脓毒症大鼠肠道菌群紊乱的影响。方法将42只SD雄性大鼠随机抽出12只为正常对照组,余下按照15 mg/kg腹腔注射内毒素方式建立内毒素脓毒症模型后,再随机分为模型组、大黄组,大黄组于制模后每日用大黄汤剂灌胃3次,模型组每日用相同剂量生理盐水灌胃3次,10天后用强迫排便法取各组大鼠新鲜大便进行乳酸杆菌、双歧杆菌、肠球菌、大肠杆菌4种菌的培养与计数,菌落计数以LgCFU/g计算。结果模型组与正常组相比较存在明显菌群紊乱,厌氧菌减少(P<0.05),需氧菌增多(P<0.05),厌氧/需氧比值降低(P<0.05);大黄组与模型组比较,有着明显的纠正菌群紊乱的作用,表现在厌氧菌增多(P<0.05),厌氧/需氧比值升高(P<0.05)。结论①脓毒症大鼠存在肠道菌群紊乱;②中药大黄有明显的纠正菌群紊乱的作用。

Objective To investigate the effects of Chinese rhubarb on intestinal flora disturbance of septic rats. Methods The male rats were divided into three groups ：normal group , model group, Chinese rhubarb group. After making the septic models induced by LPS, Chinese rhubarb was given to the rats of Chinese rhubarb group by stomach tube while 0.9% nonna saline to the normal group, 10 days later, the fresh defecate of rats were collected to cultivation and count include Lactic acid bacteria, colon bacillus, intestinal aureus, escherichia coli , and the colony count was done by LgCFU/g. Results The model group compared with normal group there exist obvious intestinal flora disturbance, anaerobic bacteria reduced （ P 〈 0.05 ）, aerobic bacteria increased （ P 〈 O. 05 ）, anaerobic bacteria / aerobic bacteria ratio reduced （ P 〈 0. 05 ） ; The Chinese rhubarb group compared with model particles there exist a clear correct flora disturbance effection, anaerobic bacteria increased, anaerobic bacteria / aerobic bacteria ratio increased （ P 〈 0.05 ）. Conclusion Significative intestinal dysbateriorsis happened to septic rats. Chinese rhubarb had obvious effects to correct intestinal dysbateriorsis of septic rats.