淮阴苜蓿SSR指纹图谱的构建

Construction of fingerprinting map based on SSR for Medicago sativa Huaiyin

采用不同的取样策略,利用SSR分子标记研究淮阴苜蓿(Medicago sativa Huaiyin)遗传多样性,确定最佳取样数,在此基础上利用筛选的SSR引物构建淮阴苜蓿的指纹图谱,并对淮阴苜蓿进行品种鉴定。本研究对320株淮阴苜蓿的单株基因组DNA设置6个处理(10、20、30、40、50、60单株DNA随机等量混合),每处理8个重复,利用筛选的4对SSR引物进行遗传多样性分析。结果表明,40株单株随机DNA的混合样品的遗传多样性指数开始趋于稳定,由此取40株即可代表淮阴苜蓿群体。采用取样数为40株单株DNA混合样,利用筛选的3对SSR引物构建淮阴苜蓿指纹图谱,并对15份供试苜蓿材料进行检测,结果表明,该方法所构建淮阴苜蓿SSR指纹图谱可以用于对淮阴苜蓿品种的鉴定。

In the present study, the genetic diversity of alfalfa was investigated using SSR molecular makers and optimum number of samples was determined using different sampling strategies. Based on the opti- mum number of samples with the screened SSR primers, the fingerprint of Huaiyin alfalfa was built and the varieties of Huaiyin alfalfa were identified. Genomic DNA from 320 strains of Huaiyin alfalfa was set to six treatments （alfalfa DNA samples randomly obtained from a mixture of equal amount of 10, 20, 30, 40, 50 and 60 plants , respectively）. Each treatment has eight repeats. Genetic diversity analysis was con-ducted using four pairs of SSR primers. The results showed that the genetic diversity index of the treat-ment of andom DNA mixture from 40 individuals was stable and the fingerprint of Huaiyin alfalfa can represent the genetic diversity of Huaiyin alfalfa groups. Fifteen alfalfa materials were tested with the treatment mentioned above and the results showed that the SSR fingerprinting could be used for the identi-fication of varieties in Huaiyin alfalfa groups.