长叶点地梅愈伤组织诱导和植株再生

Callus induction and plant regeneration of Androsace longifolia

以长叶点地梅(Androsace longifolia)的无菌实生苗的下胚轴和叶片为外植体,研究不同种类、不同浓度的激素组合培养对愈伤组织诱导、芽诱导和生根的影响。结果表明,诱导下胚轴基部愈伤组织的最佳培养基为MS+0.3mg.L-1 6-BA+0.1mg.L-1 NAA,诱导率达85%;诱导叶片愈伤的最佳培养基为MS+0.02mg.L-1NAA+0.3mg.L-1 TDZ,诱导率为80%;下胚轴愈伤诱导芽的最佳培养基为MS+0.5mg.L-1 6-BA+0.2mg.L-1 NAA,分化率达92.5%;叶片愈伤诱导芽的最佳培养基为MS+1.0mg.L-1 6-BA+0.2mg.L-1 NAA,分化率达82.5%;最佳生根培养基为MS+1.0mg.L-1 IBA,生根率达100%,移栽成活率达95%。

The hypocotyls and leaves from the sterile seedlings of Androsace longifolia were taken as explants to study the effects of different hormone compositions on callus induction, bud induction and roo-ting. The optimum medium for hypocotyls callus induction was MS ＋0.3 mg· L^-1 6-BA ＋ 0.1 mg· L^-1 NAA, and the induction rate reached 85% The medium MS ＋ 0.02mg· L^-1 NAA ＋0.3 mg· L^-1 TDZ was better for leaf callus induction, and the induction rate reached 80%. The buds were induced in medium MS ＋ 0. 5 mg· L^-1 6-BA ＋ 0. 2 mg· L^-1 NAA from hypocotyls callus, and the differentiation rate reached 92.5%. The medium MS＋L 1.0 mg· L^-1 6-BA ＋ 0.2 mg· L^-1 NAA was optimal for inducing buds from leaf callus, and the differentiation rate was the highest （82.5%）. The best rooting medium was medium MS ＋ 1.0 mg· L^-1 IBA. The rooting rate reached 100% and survival rate reached 95%.