碱裂解法快速提取番茄DNA的研究

Study on the rapid alkaline lysis method of extracting tomato DNA

以转基因番茄MM-R3a的嫩叶、子叶、下胚轴和根为试验材料,用碱裂解法快速提取番茄总DNA并用于PCR扩增.结果表明,以番茄子叶和幼嫩叶片为材料,使用该方法提取的DNA纯度和质量浓度相对较高,以稀释10倍～30倍的DNA为模板的PCR结果相对稳定.

Using tomato leaves,cotyledons,hypocotyls and roots as the materials,a simple and rapid alkaline lysis method of DNA extraction was studied and used for PCR amplification,and the results showed that cotyledons and young leaves were the appropriate materials because of the high concentrations and purity.10 times to 30 times dilution of crude DNA as template is relatively stable by PCR amplification.