豫杂一号泡桐耐盐突变体诱导及SSR分析

Induction of NaCl-tolerant mutants of Paulownia tomentosa×Paulownia fortunei and their SSR analysis

以叶片愈伤组织为材料,先分别在质量浓度为40,80,120,160,200 mg.L-1的MMS和质量分数分别为0.1%,0.2%,0.4%,0.6%的NaCl的MS增殖培养基中培养,确定愈伤组织的MMS半致死浓度和NaCl致死浓度,然后进行豫杂一号泡桐耐盐突变体的诱导及SSR分析.结果表明,MMS和NaCl处理对豫杂一号泡桐愈伤组织存活率的影响存在明显差异,叶片愈伤组织的MMS半致死剂量为120 mg.L-1,NaCl致死浓度为0.6%,连续10次继代培养,从263株筛选出的16株豫杂一号泡桐耐盐突变体的DNA碱基序列在SSR水平上呈现出显著差异.

The NaCl-tolerant mutants of Paulownia tomentosa×Paulownia fortunie and their SSR analysis were investigated based on MS media containing 40,80,120,160,200 mg·L-1 MMS for its semi-lethal dose,and quality score respectively was 0.1%,0.2%,0.4%,0.6% NaCl for its lethal concentration with the leaf calli as explants.The results indicated that there were great differences among effects of MMS and NaCl treatments on the callus survival rates,and MMS semi-lethal dose and NaCl leath concentration to the calli were 120 mg·L-1 and 0.6% respectively;The DNA base sequences of the 16 NaCl-tolerants screened out from 263 seedlings for 10 generations of subcultures continuously differed greatly at SSR level.