紫甘蓝花色苷的分离纯化

Purif ication of purple cabbage anthocyains

初步研究了紫甘蓝花色苷在SephadexLH-20凝胶层析柱上最佳的分离条件,以及在最佳条件下收集合并各组分,并进行紫外可见全扫描、薄层色谱实验。结果表明:当以30%乙醇为洗脱液,流速为1mL/min时,紫甘蓝花色苷的分离效果最好,共收集合并4个组分。紫外可见全扫描确定Ⅰ组分为非花色苷,Ⅱ、Ⅲ、Ⅳ组分为花色苷,薄层色谱实验表明Ⅱ、Ⅲ组分分别分离出2组花色苷,Ⅳ组分分离出3组花色苷。

This work was conducted to research the condition of purification, UV-Visspectrum and thin layer chromatography, which the purple cabbage anthocyains was purifited by sephadex LH-20. It was shown that optimum purification condition was elute with 30% ethanol with a flow rate of 1 mL/min, and collected four pigments, Ⅰ pigment not belonged to anthocyains, and Ⅱ, Ⅲ, IV pigment belonged to anthocyains by UV-Visspectrum; Ⅱ,Ⅲ pigment sepatated 2 anthocyains respectively, and IV pigment sepatated 3 anthocyains by thin layer chromatography.