浸提法提取、双水相体系纯化红花红色素及稳定性研究

Extracting carthamine from carthamus tinctorius by acetone aqueous and purifying by two-phase system

对红花进行预处理后,用一定浓度的丙酮溶液提取红花红色素,将提取液加入无机盐形成双水相对红色素进行纯化。研究讨论了丙酮的浓度、提取时间、料液比对提取率的影响,并对产品稳定性进行试验。实验结果表明红色素的提取工艺为:50%的丙酮料液比1g:10mL室温下超声辅助提取60min,采用丙酮-硫酸铵双水相体系对红色素进行纯化2次,将上相中的丙酮挥发、离心得纯化产品,提取率为84.9%。稳定性试验结果表明,红花红色素对温度、光和部分金属离子敏感。

Carthamine from carthamus tinctorius pretreated was extracted by aqueous acetone. Acetone- ammonium sulfate aqueous two-phase system was used for purifying carthamin. The main factors on the extraction technology were discussed, such as acetone concentration, extraction time and power to liquid ratio. The result were as follows： aqueous acetone 50%, solid to liquid ratio 1 g：10 mL, Ultrasonic- extraction time 60 min, acetone-ammonium sulfate aqueous two-phase system was purified carthamin two times. Under the conditions, the yield was 84.9%. Stability test showed that carthamin was sensitized to temperature, light and metal ions.