荧光原位杂交技术在尿路上皮癌诊断中的应用

Application of fluorescence in situ hybridization in the diagnosis of urothelial carcinoma

目的探讨荧光原位杂交技术（FISH）在尿路上皮癌诊断中的应用价值。方法采用FISH检测100例血尿患者尿脱落细胞中第3、7、17号染色体和第9号染色体p16位点异常，以组织病理学确诊尿路上皮癌为金标准，评估FISH诊断的敏感度和特异度，并与尿细胞学检查结果进行比较。结果FISH检测和尿细胞学检查诊断尿路上皮癌的敏感度分别为82．5％和49．2％，差异有统计学意义（P〈0．05）；特异度分别为86．7％和96．6％，差异无统计学意义（P〉0．05）。结论与尿细胞学比较，FISH诊断尿路上皮癌具有较高的敏感度和相似的特异度。

Objective To evaluate the results of fluorescence in situ hybridization （FISH） for detecting urothehal carcinoma. Methods Voided urine samples from 100 patients with haematuria were analyzed by FISH and cytology; labeled probes for chromosomes 3,7,17 and 9 p16 were used to assess chromosomal abnormalities. The gold standard was pathology diagnosis. The overall sensitivity and specificity of FISH were evaluated and compared with cytology. Results The sensitivity of FISH and cytology in detection urothelial cancer were 82. 5% and 49. 2% respectively,and the specificity was 86.7% and 96. 6% respectively. There was a significant difference between the sensitivity（ P 〈0. 05 ） ,and the different between the specificity was not significant （ P 〉 0.05 ）. Conclusion Compared with urine cytology, FISH diagnosis of urothelial carcinoma with high sensitivity and a similar specificity.