前列腺癌易感性与丝裂原活化蛋白激酶激酶4基因多态性的关系

Associations between-1304 T 〉 G polymorphism in the promoter of mitogen-activated protein kinase kinase 4 gene and prostate cancer susceptibility

目的探讨苏皖地区汉族人群中丝裂原活化蛋白激酶激酶4（MKK4）基因多态性与前列腺癌（PCa）易感性的关系。方法采用病例对照研究，提取174例PCa患者和252例健康人（对照组）外周血基因组DNA，应用聚合酶链反应-连接酶特异检测技术（PCR—LDR）分析PCa患者和对照组MKK4基因-1304位点的多态性，比较不同基因型与PCa易感性的关系，并探讨吸烟、饮酒等因素在其中的影响。结果MKK4基因启动子区存在1个SNP位点（-1304T〉G），基因型分别为TT型、TG型和GG型；Logistic回归分析显示，携带TG、GG和TG＋GG基因型的个体与PCa发病风险之间无明皿相关性[比值比（OR）=0．775，95％可信区间（CI）=0．516～1．164；OR=0．650，95％CI=0．216～1．956；OR=0．763，95％CI=0．514～1．135]。在高龄（〉70岁）和非饮酒人群中，TG＋GG基因型的个体发病率明显减少，调整后的OR（95％CI）分别为0．575（0．348～0．951）、0．477（0．252～0．906）。结论苏皖地区汉族人群中MKK4基因-1304位点基因多态性对PCa易感性无明显影响．

Objective To investigate the associations between-1304T 〉 G polymorphism in the promoter of mitogen-activated protein kinase kinase 4 （MKK4） gene and prostate cancer （PCa） susceptibility in the 1tan population in Jiangsu and Anhui. Methods A case control experiment was performed, consisting of 174 cases of newly diagnosed PCa and 252 cancer-free healthy controls. The peripheral blood genome DNA was extracted. The polymorphism of MKK4-1304 locus was analyzed by polymerase chain reaction-ligase detection reaction （PCR-LDR） technique. The associations between the susceptibility to PCa and different genotypes were compared. Results TF, TG, and GG genotypes were identified in the pro- moter of MKK4 gene （-1304T/G）. Logistic regression analysis revealed there was no significant difference at MKK4-1304 site between TG, GG, and TG ＋ GG genotypes and TF genotype （OR = 0. 775, 95% CI = 0. 516-1. 164; OR =0. 650, 95% CI =0. 216-1. 956; OR =0. 763, 95% CI =0. 514-1. 135, respectively）. However, in the stratification analysis, the decreased risk of PCa was associated with the-1304G variant genotypes （ -1304TG/GG）, particularly in the subgroups of subjects older than 70 years （ OR = 0. 575, 95% CI = 0. 348-0. 951 ） and no-smokers （ OR = 0. 477, 95% CI = 0. 252-0. 906 ）. Conclusion The MKK4-1304 polymorphism is not related directly to the susceptibility to PCa in the Han population in Jiangsu and Anhui.