X盒结合蛋白1、免疫球蛋白结合蛋白在肝癌组织中的表达及意义

The expression and significance of X box binding protein 1 and immunoglobulin-binding protein in hepatocelluar carcinoma

目的检测x盒结合蛋白1（XBP1）、免疫球蛋白结合蛋白（Bip）在肝癌组织中的表达，探讨其在肝癌生长中的作用机制。方法采用逆转录-聚合酶链反应（RT—PCR）法检测术中所取的42例肝癌标本、15例同个体癌旁1．0cm肝组织和15例正常肝组织中XBP1、Bip的mRNA表达。应用Westernblot法验证57例肝癌组织和正常肝组织标本中XBP1、Bip在蛋白水平的表达。结果肝癌组织、癌旁肝组织、正常肝组织中XBP1mRNA相对表达量分别为0．4396±0．0241、0．4152±0．0252、0．4095±0．0149，XBP1mRNA在3组之间的表达呈下降趋势（P〈0．05）；肝癌组织、癌旁肝组织、正常肝组织中BipmRNA相对表达量分别为0．4772±0．0401、0．4332±0．0488、0．4301±0．0398，BipmRNA在3组之间的表达呈下降趋势（P〈0．05）。实验对肝癌组织和正常肝组织中XBP1和Bip的蛋白表达进行了定性验证：在肝癌组织和正常肝组织中均有两种蛋白的表达，但肝癌组织中的表达明显高于正常肝组织。结论在肝癌的生长过程中存在未折叠蛋白反应（UPR）的激活。在肝癌组织中XBP1、BipmRNA和蛋白的表达一致，均呈高表达。这些基因和蛋白表达的改变可能与肝癌的发生、生长及转移密切相关。

Objective To detect the express of X box binding protein 1 （XBP1） and immunoglobulin-binding protein （Bip） in the tissue of hepatocellular carcinoma, demonstrating that it exist activation of unfold protein response （UPR） in process of growth, and the expression of UPR was higher than normal tissue; approaching the mechanism of action in the growth of hepatoma, searching the new target for the therapy of hepatocellular carcinoma. Methods To study the mRNA of XBP1, Bip example of hepatome, 15 cases paired adjacent hepatic tissues to carcinoma and 15 cases normal hepatic tissues by reverse transcription-polymerase chain reaction （RT-PCR）. To qualitation check the expression of XBP1 and Bip in the level of protein in 57 cases of bepatome and normal hepatic tissues by Western blotting. Results The express of XBP1 mRNA in hepatoma tissue, adjacent hepatic tissue to carcinoma and normal hepatic tissue respectively was 0. 4396 ±0. 0241, 0. 4152 ±0. 0252, 0. 4095 ±0. 0149;The express of XBP1 mRNA was higher in hepatoma than others （P 〈 0. 05）. The express of Bip mRNA in hepatoma tissue, adjacent hepatic tissue to carcinoma and normal hepatic tissue respectively was 0. 4772 ± 0. 0401, 0. 4332 ± 0. 0488, 0. 4301 ± 0. 0398 ;the express of Bip mRNA was higher in hepatoma than others （P 〈 0. 05 ）. The experiment indicate：the two protein all express in the hepatoma tissue and normal hepatic tissue, but they were higher in hepatoma tissue than in the normal hepatic tissue. Conclusion The activation of UPR exists in process of growth. The express of XBP1, Bip, protern and their mRNA was accordant, all of them were higher in hepatoma tissue than in normal hepatic tissue. The changes of these gene and protern is possible to have the relationship with the carcinogenesis, growth and transfer of hepatoma. And it may have significant effect in molecule mechanism of hepatoma. It also descriptes that unfold protein response may have very important role in the pocess of carcinogenesis.