血红素氧合酶在糖尿病大鼠阴茎海绵体的表达

Expression of heme oxygenase in corpus cavernosum of diabetic rats

目的检测血红素氧合酶1和2（HO-1／HO-2）在糖尿病（DM）大鼠阴茎海绵体（CC）的表达变化，探讨其在糖尿病相关勃起功能中的作用。方法SD雄性大鼠50只，随机取30只制作糖尿病模型，余20只为正常对照。4周和8周后用阿扑吗啡试验评价大鼠勃起功能；免疫组织化学法及Western blot法检测HO-1、HO-2在大鼠CC内的表达部位及水平。结果DM大鼠勃起次数在4周（2．17±0．94）和8周（0．85±1．07）时与对照组[4．0±1．15（4周）；4．2±1．32（8周）]比均下降（P〈0．01），HO-1在4周（32．87±3．22）和8周（20．65±2．34）时的表达高于相应对照组[13．52±3．25（4周）；12．35±2，29（8周）]，P〈0．01，但8周较4周表达降低（P〈0．01），HO-2在4周（14．32±1．21）和8周（8．82±2．35）时的表达均低于相应对照组[20．91±2．07（4周）；21．02±2．10（8周）]，P〈0．01，且随时间逐渐加重（P〈0．01）；对照组间差异无统计学意义（P〉0．05）。结论糖尿病造成大鼠勃起功能下降，可能与HO存DM大鼠CC内的表达降低密切相关。

Objective To detect the expression of hem-e oxygenase 1 and 2 in the corpus cavernosum of diabetic rats and explore their significance in diabetic erectile function. Methods Of 50 Sprague- Dawley male rats, 30 rats were selected randomly, and induced into diabetes mellitus model, and the rest 20 rats served as normal control group. Four weeks and 8 weeks later, erectile function of each rat was accessed by apomorphine assay. The distribution and content of HO-1 and HO-2 in the penile tissues were determined by using immunohistochemistry and Western blotting. Results The erection times of DM rats were decreased after 4 weeks （2. 17 ± 0. 94） and 8 weeks （0. 85 ± 1.07 ） compared to normal controls [ 4. 0 ± 1.15 （4 weeks）, P 〈 0. 01 ; 4. 2 ± 1.32 （8 weeks） ,P 〈 0. 01 ] ; the expression of HO-1 in DM rats was increased after 4 weeks （ 32. 87 ± 3.22） and 8 weeks （20. 65 ± 2. 34） compared to normal controls [13.52±3.25 （4 weeks）,P〈0.01; 12. 35 ±2.29 （8 weeks）,P〈0.01], and the expression after 8 weeks was lower than that after 4 weeks （ P 〈 0.01 ） ; the expression of HO-2 in DM rats was declined after 4 weeks （ 14. 32 ± 1.21 ） and 8 weeks （ 8.82 ± 2. 35 ） compared to that in the respective normal controls [ 20. 91 ± 2. 07 （4 weeks ）, P 〈 0. 01 ; 21.02 ± 2. 10 （ 8 weeks ）, P 〈 0.01 ], and the change enlarged along with time （P 〈0.01 ） , but the difference in the normal controls had no statistically significant difference （ P 〉 0. 05 ）. Conclusion Diabetes mellitus can decrease the erectile function of rats, which may be related to the decrease of HO expression in the corpus cavernosnm of diabetic rats.