间接竞争酶联免疫法检测水样中的重金属镉

Development of heavy metal cadmium in water samples by indirect competitive enzyme-linked immunosorbent assay

利用重金属镉多克隆抗体,建立一种低仪器成本的检测水样中重金属镉的间接竞争酶联免疫法,其检测限(IC15)为0.76μg/L,灵敏度(IC50)为11.33μg/L。交叉反应结果表明,该抗体除与汞螯合物的交叉反应率为10.9%,与其他金属(锰,铬,镁,铁,铅,镍,银和铜)螯合物的交叉反应率均低于1.32%。自来水和河水样品中镉的添加回收率为93.95%～107.40%,变异系数为3.97%～14.69%。

An indirect competitive enzyme-linked immunosorbent assay was developed to low instrument cost detect cadmium in water samples using the polyclonal antibody.The limit of detection（IC15） was 0.76μg/L,and the sensitivity（IC50）was 11.33μg/L.The cross-reactivity rates of the antibody with other metals（Mn,Cr,Mg,Fe,Pb,Ni,Ag and Cu）chelates were below 1.32%,except for Hg-chelate with a cross-reactivity rate 10.9%.The recoveries of cadmium in spiked tap water and river water were 93.95%～107.40%,and the coefficients of variation were 3.97%～ 14.69%.