大孔树脂分离纯化苦苣菜黄酮的工艺研究

Study on separation and purification of Sonchus oleraceus L total flavonoids by AB-8 macroporous resin with response surface analysis

研究大孔树脂纯化苦苣菜黄酮的条件。利用静态吸附方法筛选纯化苦苣菜黄酮的最适大孔树脂,利用动态吸附方法研究最适大孔树脂纯化苦苣菜黄酮的条件。结果表明,大孔树脂AB-8对吸附苦苣菜黄酮的效果最好,最佳纯化条件:上样液浓度为3.73%,上样液速率为3.6mL/min,上样液pH 5.18;用78.20%的乙醇溶液、以120mL 2.88mL/min的速率洗脱。利用大孔吸附树脂AB-8在上述最佳条件下,吸附率可达84.32%;解吸率91.73%。

To study the purification conditions with macroporous resin in Sonchus culture.Using the static adsorption method for screening optimum macroporous resin to be purification of Sonchus oleraceus flavones,using dynamic adsorption method to study optimum macroporous resin conditions of purification of Sonchus oleraceus flavonoid.Results the macroporous resin adsorption of AB-8 on Sonchus flavone was the best,the best purification conditions were： 3.73% sample concentration,sample liquid rate of 3.6 mL / min,sample liquid pH was 5.18;by 78.20% ethanol solution,with 120 mL（column volume） 2.88 mL / min rate elution.Using macroporous adsorption resin AB-8,under the optimum conditions,the adsorption rate is up to 84.32%;desorption rate 91.73%.