在PVDF-SiO_2复合微球上的脂肪酶固定化

Immobilization of Lipase on PVDF-SiO_2 Composite Microspheres

采用相转移法制备了包裹SiO2的聚偏氟乙烯(PVDF)复合微球,用其固定化假丝酵母脂肪酶(CRL).结果表明,纯PVDF球、PVDSiO2复合球用作酶固定化载体,对CRL的吸附量都较大.随着PVDFSiO2复合球中SiO2含量增多,其酶吸附量和活力回收率都有提高.PVDF与SiO2质量比为1:1的复合球的酶活力回收率最高,可达50.98%.固定化CRL后,PVDFSiO2复合球比SiO2更易实现固液分离,且对底物溶液无污染.

Polyvinylidene fluoride （PVDF）-SiO2 composite microspheres enveloping SiO2 were prepared by phase transition process and used as cartier to immobilize Candida rugosa lipase （CRL）. It is found that CRL immobilized on PVDF or PVDF-SiO2 composite microspheres has a large loading. As to PVDF-SiO2 composite microspheres, with the increase of SiO2 amount, both the loading and recovery rate of activity increase. When the mass ratio of PVDF to SiO2 is 1：1, they have the maximal loading of 200 mg/g and the maximal recovery rate of activity of 50.98%, the PVDF-SiO2 composite microspheres are the optimized carrier in the experiment. Comparing PVDF-SiO2 composite with SiO2, the former is superior in filtration and has no pollution on substrate solution.