中药北刘寄奴中黄酮类成分的分离与鉴定

Isolation and identification of flavonoids from Siphonostegia chinensis Benth.

目的研究中药北刘寄奴(Siphonostegia chinensis Benth.)的化学成分。方法采用正相硅胶、反相ODS、Sephadex LH-20等柱色谱及高效液相色谱等手段进行分离纯化,并通过理化性质与光谱分析方法鉴定了化合物的结构。结果从北刘寄奴体积分数为95%的乙醇提取物中分离鉴定了8个黄酮类单体成分,分别为芹菜素(apigenin,1)、芹菜苷(apigentrin,2)、木犀草素(luteolin,3)、木犀草素-7-O-β-D-葡萄糖苷(luteolin-7-O-β-D-glucopyranoside,4)、槲皮素-3-O-β-D-葡萄糖苷(quer-cetin-3-O-β-D-glucopyranoside,5)、槲皮素-3-O-α-L-鼠李糖苷(quercetin-3-O-α-L-rhamnopyranoside,6)、isoscutellarein 8-glucuronide(7)、大豆素(daidzein,8)。结论其中化合物4～8为首次从阴行草属中分离得到。

Objective To study the chemical constituents from Siphonostegia chinensis Benth. Methods The compounds were isolated by chromatographic, ways such as silica gel, ODS, Sephadex LH-20 and HPLC, and identified by the chemical and physical methods, especially spectral analysis. Results Eight flavonoids were i- solated. The structures of them were identified as apigenin （ 1 ）, apigentrin （2）, luteolin （3）, luteolin-7-O-β- D-glucopyranoside （4）, quercetin-3-O-β-D-glucopyranoside （ 5 ）, quercetin-3-O-β-L-rhamnopyranoside （ 6 ）, isoscutellarein 8-glucuronide （7）and daidzein （8）. Conclusions Among them ,4-8 are obtained from Siphono- stegia genus for the first time.