水产品中甲醛HPLC测定的前处理方法探讨

Study on pre-treatment methods for determination of formaldehyde in aquatic products by HPLC

为了探讨前处理方法对水产品甲醛检测的影响,本文比较了三氯乙酸结合超声提取法(超声+TCA法)、三氯乙酸提取法(TCA法)、蒸馏水提取法、水蒸气蒸馏法四种前处理方法的高效液相色谱法(HPLC)测定甲醛。结果显示,水蒸气蒸馏和三氯乙酸超声提取平均回收率分别为109.06%和103.62%,而蒸馏水提取的回收率低于40%。经优化后的三氯乙酸超声提取条件优化为超声时间25min,10%TCA加入量10mL。因此,三氯乙酸超声提取具有稳定性较好,操作简便,与水蒸气蒸馏法前处理效果相当,是一种适合用于大多数鲜活和冷冻水产品甲醛含量检测的前处理方法。

In order to investigate the effects of pre-treatment methods on determination of formaldehyde in food,the experiment,four pre-treatment methods,trichloroacetic acid combined with ultra-sonic extraction（ultra-sonic＋TCA）,trichloroacetic acid solution extraction（TCA）,water extraction and steam distillation was compared in determination of formaldehyde by high performance liquid chromatography（HPLC）.The results showed that the average recovery of steam distillation and ultra-sonic＋TCA method were 109.06% and 103.62%,respectively,whereas,water extraction was of less than 40%.The optimized conditions of TCA combined with ultra-sonic were ultrasonic time of 25min and 10%TCA addition value of 10mL.Therefore,TCA combined with ultra-sonic exhibited similar result in extraction of formaldehyde compared with steam distillation.The method with simple operation,and good stability,could be applied to determine the formaldehyde in fresh and frozen aquatic products.