海蓬子正丁醇萃取物的体外抗氧化性研究

Antioxidant activity of butylalcohol extracts in Salicornia herbacea in vitro

对海蓬子的正丁醇萃取物,通过硅胶柱层析、葡聚糖凝胶LH-20柱层析、高效液相色谱分离进行纯化,得到一种活性成分SBF;通过LC-MS检测,确定其分子量为802。并对SBF的还原能力、对羟自由基、DPPH自由基的清除能力和对脂质过氧化的抑制能力进行检测,研究其体外抗氧化活性。研究结果表明,SBF具有一定还原能力,对羟自由基、DPPH自由基都有一定的清除能力,但均小于阳性对照VC;SBF对脂质过氧化也具有一定抑制作用,在浓度小于1.2mg/mL时,抑制作用大于VC,在浓度大于1.2mg/mL时,抑制作用小于VC。

Separation and purification of Salicornia herbacea butylalcohol fractions（SBF）were investigated.One active ingredient could be obtained by silica gel column chromatography,Sephadex LH-20 gel filtration chromatography and high performance liquid chromatography.The molecular weight of this active ingredient was 802,detected by LC-MS.In vitro,antioxidant activity of Salicornia herbacea butylalcohol fractions（SBF）were studied by assaying reducing power,scavenging ability of SBF on the hydroxyl radical,DPPH and inhibition of lipid peroxidation.There was scavenging ability of SBF on the hydroxyl radical.DPPH and reducing power,but less than the positive control VC.SBF had a certain inhibition of lipid peroxidation,in the concentration of less than 1.2mg/mL,the inhibition was greater than VC,the concentration of more than 1.2mg/mL,the inhibition was less than VC.