微生物脂肪酶的研究与应用

Research and applications of microbial lipases

脂肪酶是一类能够催化酯的水解反应以及在非水相体系中催化脂肪酸和醇类发生酯化反应的酶类。随着酶学技术的快速发展,微生物脂肪酶也受到了越来越多的关注。作为生物催化剂,脂肪酶一直以来都是生物技术领域中最重要的一类酶。本文探讨了脂肪酶的来源、理化性质、脂肪酶活力测定,同时对脂肪酶的非水相催化特性以及脂肪酶在食品工业,医药、洗涤剂、皮革、造纸和生物柴油工业领域中的应用进行了讨论。

Lipase is a kind of enzymes which catalyse the hydrolysis of esters and esterification of fatty acid and alcohol.Lipases constitute the most important group of biocatalysts for biotechnological applications.This review described physicochemical origin and properties of lipases,lipase activity determination,catalytic properties of lipases in nonaqueous phase and various industrial applications of microbial lipases in the food,pharmaceuticals,detergent,leather,papermaking and biodiesel.