RP-HPLC法测定北豆根药材中5个生物碱的含量

RP-HPLC simultaneous determination of 5 alkaloids in Rhizoma Menispermi

目的:采用RP-HPLC法同时测定中药材北豆根中青藤碱、蝙蝠葛苏林碱、蝙蝠葛诺林碱、蝙蝠葛新诺林碱和蝙蝠葛碱5种生物碱的含量。方法:采用色谱柱Welchrom-C18(250 mm×4.6 nm,5 mm),以甲醇(A)-乙腈(B)-0.4%二乙胺水溶液(C)为流动相,梯度洗脱[0～13 min,A-B-C(18:20:62);13～45 min,A-B-C(18:20:62)→A-B-C(37:20:43);45～60min,A-B-C(37:20:43)];流速1.0 mL·min-1;检测波长为285 nm。结果:青藤碱、蝙蝠葛苏林碱、蝙蝠葛诺林碱、蝙蝠葛新诺林碱和蝙蝠葛碱5种生物碱的线性范围分别为0.016～0.2μg(r=0.9995),0.029～0.363μg·mL-1(r=0.9996),0.09～1.125μg·mL-1(r=0.9996),0.336～4.2μg·mL-1(r=0.9997)与0.172～2.15μg·mL-1(r=0.9996);加样同收率(n=6)分别为99.2%,98.5%,102.2%,96.5%和98.4%。结论:该方法简便、快速、准确,重复性好,可以用于北豆根药材及其制剂的含量测定。

Objective：To establish an RP-HPLC method for simultaneous determination of 5 alkaloids（sinomenine,daurinoline,dauricinolone,daurisoline,dauricine） in Rhizoma Menispermi from different sources.Methods：The reversed phase HPLC system containing of a Welchrom-C18 column（250 mm×4.6 mm,5 μm） was adopted with a mixture liquid of（A）-acetonitrile（B） and 0.4% diethylamine water solution（C） as mobile phase in a gradient elution at the flow rate of 1.0 mL·min-1,the detection wavelength was 285 nm and the column temperature was 40 ℃.Results：The linear ranges were 0.016-0.2 μg（r=0.9995）,0.029-0.363 μg·mL-1（r=0.9996）,0.09-1.125 μg·mL-1（r=0.9996）,0.336-4.2 μg·mL-1（r=0.9997） and 0.172-2.15 μg·mL-1（r=0.9996）,respectively.The average recoveries（n=6） were 99.2%,98.5%,102.2%,96.5% and 98.4%,respectively.Conclusion：The validated HPLC method has the advantages of precision,reproducibility and reliability,allowing simultaneous determination of alkaloids in Rhizoma Menispermi and it＇s production.