荧光探针测定雷尼替丁、尼扎替丁和西咪替丁

Fluorescence probe method for determination of ranitidine,nizatidine and cimetidine

目的:建立一种测定雷尼替丁、尼扎替丁和西咪替丁3种H2组胺受体药物的高灵敏度的荧光探针新方法。方法:本方法是基于小檗碱和葫芦[7]脲生成稳定的包合物并使其荧光强度显著增强,当加入3种药物中的任何一种均能使葫芦[7]脲/小檗碱包合物的荧光显著猝灭。据此建立了一种以小檗碱为荧光探针测定3种H2组胺受体药物的新方法。结果:药物在一定的浓度范围内与其相应的荧光猝灭值ΔF之间呈良好的线性关系,雷尼替丁、尼扎替丁和西咪替丁检测限分别为0.007,0.007,0.020μg·mL-1,比一般的光谱方法高2个数量级。同时对药物制剂和加标尿样进行测定,获得了满意的回收率。结论:该方法可用于药物制剂和生物体液中上述3种H2组胺受体药物的测定。

Objective：To establish a new method for determination of ranitidine,nizatidine and cimetidine by using sensitive fluorescence probe.Methods：CB[7] could react with berberine to form a stable complex,and the fluorescence intensity of the complex was greatly enhanced.Ranitidine,nizatidine and cimetidine can dramatically quench the fluorescence intensity of the complex.Accordingly,a new fluorescence quenching method was established for the determination of these three histamine H2 receptor antagonists.Results：There was a good linear relationship between fluorescence quenching values（ΔF） and a certain concentration range of medicament concentration.The minimal detection limits of ranitidine,nizatidine and cimetidine respectively was 0.007,0.007 and 0.020 μg·mL-1,respectively,which was 2 orders of magnitude higher than normal spectroscopic method.Pharmaceutical preparations and spiked urine samples were also determined and satisfactory recoveries were obtained.Conclusion：The method can be applied to determination of ranitidine,nizatidine and cimetidine in both pharmaceutical samples and urine samples.