黄芪药材HPLC特征图谱研究(1)皂苷类成分

HPLC characteristic spectrum analysis of saponins in astragali radix

目的:建立黄芪中皂苷类成分的液相色谱特征图谱。方法:通过比较碱化处理前后皂苷类成分的变化,进一步证明碱性条件可以促使黄芪皂苷Ⅰ、Ⅱ转化为黄芪皂苷Ⅳ,并确定黄芪皂苷Ⅰ、Ⅱ、Ⅲ、Ⅳ为特征成分。结果:4个特征成分分离良好。结论:本方法简便易行,所选特征成分可用于黄芪中皂苷类成分的检查。

Objective：To establish the method of characteristic speetrum on astragali radix for saponins by HPLC.Methods：AstragalosideⅠ,Ⅱcould be transformed to astragaloside Ⅳ by Alkaline treatment.and astragalosideⅠ,Ⅱ,Ⅲ,Ⅳ were proved to be characteristic components of astragali radix.Results：The four saponins could be detected respectively.Conclution：This is a simple method which can be used to control the quality of astragali radix.